Use of an alumina column in estimating total iron-binding capacity.
نویسنده
چکیده
I describe a simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron. This technique involves use of an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid. This method, when compared with the magnesium carbonate method of Ramsay (Clin. Chim. Acta 2: 221, 1957) by regression analysis and tests of precision, showed a negative bias for results by the alumina technique with respect to the magnesium carbonate method.
منابع مشابه
Effects of Alpha-Lipoid Acid on Serum Iron, Total Iron Binding Capacity and Hematological Indices in β-Thalassemia Major Patients: A Cross Over Clinical Trial
Background and Objectives: Accumulation of iron is the most common consequences of repeated blood transfusions in β-thalassemia major patients. Iron chelating effects of alpha-lipoic acid have been reported by several studies. The aim of this study was to investigate effects of supplementation with alpha-lipoic acid, as a chelator, on serum iron and hematological indices in these patients. Ma...
متن کاملInvestigating Changes in the Serum Iron, TIBC (Total Iron Binding Capacity) and Transferrin Levels in Patients with Morbid Obesity Undergoing Bariatric Surgery in Kerman in 2018: A Short Report
Background and Objectives: Bariatric surgery is one of the most effective treatments for obesity. This study was designed to investigate changes in the serum iron, TIBC (Total Iron Binding Capacity) and transferrin levels in patients with morbid obesity undergoing bariatric surgery in Kerman in 2018. Materials and Methods: This prospective study was carried out on 65 participants who had gone ...
متن کاملDesferrioxamine provocative test: methodology for estimating iron and total iron binding capacity.
To the Editor: In our report entitled “Treatment of Cooley’s Anemia” (Blood 76:435, 1990), we described a desferrioxamine provocation test in which we measure serum iron and iron binding capacity following an infusion of the chelator. In the report, we failed to state clearly that we do not use standard colorimetric tests to make these measurements. The mild reducing agents used in most colorim...
متن کاملAssociation between helicobacter pylori infection and serum iron profile
Background: It is clear that Helicobacter pylori (H. pylori) is a spiral bacterium that infects human and causes gastritis and peptic ulcer. The aim of this study was to determine the relationship between H .pylori infection and the iron status of the body. Methods: This study was conducted in Shahid Beheshti Hospital of Babol University of Medical Sciences, Babol, Iran from August 2007 to July...
متن کاملOptimization of Dynamic Binding Capacity of Anion Exchange Chromatography Media for Recombinant Erythropoietin Purification
Background:The dynamic binding capacity (DBC) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. Optimization of the process criteria for maximum DBC avoids extra process scale-up and reduces ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 26 1 شماره
صفحات -
تاریخ انتشار 1980